Orazaliyeva, Madina2024-06-252024-06-252023-04-25Orazaliyeva, M. (2023). Electroporation as a transfection method for MB49 urothelial carcinoma cell line. Nazarbayev University School of Medicinehttp://nur.nu.edu.kz/handle/123456789/8006In recent years, periostin has gained research interest as a possible prognostic biomarker tool for many cancer types due to its strong correlation with poor prognosis and metastasis. However, in the case of bladder cancer, the physiological role of periostin in its development and progression remains poorly understood. To explore this further, transfection, a research strategy commonly used to examine certain gene functions, was performed. In this study, the MB49 cells, a murine urothelial carcinoma cell line, were transiently transfected with periostin DNA in vitro via electroporation. The study aimed to determine whether electroporation is more effective for transfecting MB49 cells with periostin plasmid than chemical reagents. The results showed that electroporation efficiency was low relative to transfection efficiency obtained using chemical reagents, with a transfection efficiency rate of only 4.22%. This finding suggests that electroporation is not an effective method for transient transfection of MB49 cells with periostin plasmid in vitro compared to transfection using cationic lipid reagents, highlighting the limitations of this transfection method. In future studies, alternative physical and non-physical transfection techniques should be explored to improve the transfection efficiency of MB49 cells.enAttribution-NonCommercial-NoDerivs 3.0 United StatesType of access: EmbargoBachelor's thesis