ZD7288 enhances long-term depression at early postnatal medial perforant path-granule cell synapses
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Date
2012-05-08
Authors
Guli, Xiati
Tokay, Tursonjan
Rohde, Marco
Bender, Roland A.
Kohling, Rudiger
Kirschstein, Timo
Journal Title
Journal ISSN
Volume Title
Publisher
Neural Plasticity
Abstract
Hyperpolarization-activated, cyclic nucleotide-gated nonselective (HCN) channels modulate both membrane potential and resistance and play a significant role in synaptic plasticity. We compared the influence of HCN channels on long-term depression
(LTD) at the medial perforant path-granule cell synapse in early postnatal (P9–15) and adult (P30–60) rats. LTD was elicited
in P9–15 slices using low-frequency stimulation (LFS, 900 pulses, 1Hz; 80 ± 4% of baseline). Application of the specific HCN
channel blocker ZD7288 (10 μM) before LFS significantly enhanced LTD (62 ± 4%; P < 0.01), showing HCN channels restrain LTD induction. However, when ZD7288 was applied after LFS, LTD was similar to control values and significantly different from
the values obtained with ZD7288 application before LFS (81 ± 5%; P < 0.01), indicating that HCN channels do not modulate
LTD expression. LTD in slices from adult rats were only marginally lower compared to those in P9–15 slices (85 ± 6%), but bath
application of ZD7288 prior to LFS resulted in the same amount of LTD (85 ± 5%). HCN channels in adult tissue hence lose their modulatory effect. In conclusion, we found that HCN channels at the medial perforant path-granule cell synapse compromise
LFS-associated induction, but not expression of LTD in early postnatal, but not in adult, rats.
Description
Keywords
depression, nucleotide-gated nonselective, cell synapse, Research Subject Categories::MEDICINE
Citation
Xiati Guli, Tursonjan Tokay, Marco Rohde, Roland A. Bender, Rüdiger Köhling, and Timo Kirschstein, “ZD7288 Enhances Long-Term Depression at Early Postnatal Medial Perforant Path-Granule Cell Synapses,” Neural Plasticity, vol. 2012, Article ID 237913, 9 pages, 2012. doi:10.1155/2012/237913