DETERMINATION OF ANTIBACTERIAL SENSITIVITY OF BACTEROIDES FRAGILIS ISOLATED FROM INTRAABDOMINAL INFECTIONS
No Thumbnail Available
Date
2020
Authors
Kozhakhmetova, S.
Zholdybayeva, E.
Atavliyeva, S.
Tarlykov, P.
Mukhtarova, K.
Syzdykov, T.
Khasenov, R.
Ramankulov, E.
Journal Title
Journal ISSN
Volume Title
Publisher
International conference "MODERN PERSPECTIVES FOR BIOMEDICAL SCIENCES: FROM BENCH TO BEDSIDE”; National Laboratory Astana
Abstract
Introduction: Modern intra-abdominal infections are characterized by rapidly growing antibiotic resistance.
Gram-negative bacillus B.fragilis is one of the dominating anaerobic pathogens. Thus this research
aims to study sensitivity of B.fragilis, isolated from patients’ peritoneal exudate , to antibacterial drugs.
Methods: Clinical samples from 72patients from the surgical department of the City Hospital No. 1and
the Multidisciplinary Regional Hospital No. 2(Nur-Sultan) from 2018 to 2019 were collected to form B.fragilis
collection. All isolated bacteroid strains were identified using MALDI-TOF MS (with a score of ≥2) and
via identification of a direct nucleotide sequence of the 16SrRNA gene fragment. Minimum inhibitory
concentration (MIC) of antibiotics was determined using M.I.C. strips (Oxoid, England) with antibiotic
concentration gradients plotted. Four antimicrobial agents were tested: ciprofloxacin, metronidazole,
meropenem and clindamycin.
Results: Overall, 9 strains of anaerobic bacteria B.fragilis were isolated from 72clinical samples of various
intra-abdominal infections. It was found that in intraabdominal infections after B.fragilis extraction,
often B. thetaiotaomicron, Parabacteroides distasonis, B. ovatus, less often B. clarus, Prevotella heparinolytica
and B.salyersiae are extracted. After antibiotic sensitivity test of B. fragilis, it is found that most
of the studied cultures (67%) are ciprofloxacin resistant and smaller part (14%) is resistant to metronidazole.
25% of the strains showed moderate sensitivity to meropenem, 33% to ciprofloxacin, 50% to clindamycin
and 57% to metronidazole. 29% of the strains demonstrated high sensitivity to metronidazole and
50% to clindamycin. The highest sensitivity of B. fragilis (75%) was to meropenem (carbapenem) among
all antimicrobial agents tested.
Conclusions: Thus, obtained results help to elucidate inclusion of drugs that are the most active against
the B.fragilis. Namely, inclusion of meropenem as monotherapy and metronidazole in combination with
other antibiotics for the treatment of intra-abdominal infections.
Description
Keywords
B.fragilis, antibiotic resistance, peritonitis, Research Subject Categories::MEDICINE