GENETIC DIVERSITY OF FRANCISELLA TULARENSIS SUBSP. HOLARCTICA IN KAZAKHSTAN

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dc.contributor.authorShevtsov, Vladislav
dc.contributor.authorKairzhanova, Alma
dc.contributor.authorShevtsov, Alexandr
dc.contributor.authorShustov, Alexandr
dc.contributor.authorKalendar, Ruslan
dc.contributor.authorAbdrakhmanov, Sarsenbay
dc.contributor.authorLukhnova, Larissa
dc.contributor.authorIzbanova, Uinkul
dc.contributor.authorRamankulov, Yerlan
dc.contributor.authorVergnaud, Gilles
dc.date.accessioned2021-09-16T10:02:11Z
dc.date.available2021-09-16T10:02:11Z
dc.date.issued2021-05-17
dc.description.abstractTularemia is a highly dangerous zoonotic infection due to the bacteria Francisella tularensis. Low genetic diversity promoted the use of polymorphic tandem repeats (MLVA) as first-line assay for genetic description. Whole genome sequencing (WGS) is becoming increasingly accessible, opening the perspective of a time when WGS might become the universal geno-typing assay. The main goal of this study was to describe F. tularensis strains circulating in Kazakhstan based on WGS data and develop a MLVA assay compatible with in vitro and in silico analysis. In vitro MLVA genotyping and WGS were performed for the vaccine strain and for 38 strains isolated in Kazakhstan from natural water bodies, ticks, rodents, carni-vores, and from one migratory bird, an Isabellina wheatear captured in a rodent burrow. The two genotyping approaches were congruent and allowed to attribute all strains to two F. tularensis holarctica lineages, B.4 and B.12. The seven tandem repeats polymorphic in the investigated strain collection could be typed in a single multiplex PCR assay. Identical MLVA genotypes were produced by in vitro and in silico analysis, demonstrating full compatibility between the two approaches. The strains from Kazakhstan were compared to all pub-licly available WGS data of worldwide origin by whole genome SNP (wgSNP) analysis. Genotypes differing at a single SNP position were collected within a time interval of more than fifty years, from locations separated from each other by more than one thousand kilo-meters, supporting a role for migratory birds in the worldwide spread of the bacteria.en_US
dc.identifier.citationShevtsov, V., Kairzhanova, A., Shevtsov, A., Shustov, A., Kalendar, R., Abdrakhmanov, S., Lukhnova, L., Izbanova, U., Ramankulov, Y., & Vergnaud, G. (2021). Genetic diversity of Francisella tularensis subsp. holarctica in Kazakhstan. PLOS Neglected Tropical Diseases, 15(5), e0009419. https://doi.org/10.1371/journal.pntd.0009419en_US
dc.identifier.issn1935-2727
dc.identifier.urihttps://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0009419
dc.identifier.urihttps://doi.org/10.1371/journal.pntd.0009419
dc.identifier.urihttp://nur.nu.edu.kz/handle/123456789/5800
dc.language.isoenen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.ispartofseriesPLOS Neglected Tropical Diseases;https://doi.org/10.1371/journal.pntd.0009419 May 17, 2021
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectType of access: Open Accessen_US
dc.subjectFrancisella tularensisen_US
dc.subjectGenotypingen_US
dc.subjectKazakhstanen_US
dc.subjectPhylogeographyen_US
dc.subjectSingle nucleotide polymorphismsen_US
dc.subjectAnimal migrationen_US
dc.subjectGenomicsen_US
dc.subjectTularemiaen_US
dc.titleGENETIC DIVERSITY OF FRANCISELLA TULARENSIS SUBSP. HOLARCTICA IN KAZAKHSTANen_US
dc.typeArticleen_US
workflow.import.sourcescience

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