CD320 as a potential clinical biomarker for GOF p53 mutants in non-small cell lung cancer

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dc.contributor.authorTolymbekova, Ainur
dc.date.accessioned2024-05-14T07:12:10Z
dc.date.available2024-05-14T07:12:10Z
dc.date.issued2024-04-26
dc.description.abstractThe CD320 is the receptor responsible for the uptake of vitamin B12 bound to transcobalamin in humans. Due to the heavy reliance on DNA replication, proliferating cells require increased uptake of vitamin B12. Indeed, increased expression of CD320 was observed in many types of cancer, including lung cancer. The unpublished ribosome profiling data indicated increased ribosome protected fragments to mRNA ratio for CD320 in p53 gain-of-function mutant non-small cell lung cancer (NSCLC) cells compared to ones with wild-type p53. That evidence might point to the association between CD320 expression and p53 status. Specifically, it was hypothesized that GOF p53 mutant protein is associated with increased translation of CD320 protein in NSCLC cells. Aim of the study was to induce the TP53 knockout using CRISPR-Cas9 system in three NSCLC cell lines with different p53 status, isolate colonies with 100% KO pull, and assess the expression of CD320 on mRNA and protein level before and after the p53 knockout.en_US
dc.identifier.citationTolymbekova, Ainur. (2024) CD320 as a potential clinical biomarker for GOF p53 mutants in non-small cell lung cancer. Nazarbayev University School of Medicineen_US
dc.identifier.urihttp://nur.nu.edu.kz/handle/123456789/7661
dc.language.isoenen_US
dc.publisherNazarbayev University School of Medicineen_US
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/*
dc.subjectType of access: Restricteden_US
dc.subjectCD320en_US
dc.subjectCRISPR-Cas9en_US
dc.titleCD320 as a potential clinical biomarker for GOF p53 mutants in non-small cell lung canceren_US
dc.typeMaster's thesisen_US
workflow.import.sourcescience

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