Acceleration of Skin Wound-Healing Reactions by Autologous Micrograft Tissue Suspension
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Date
2020-06
Authors
Jimi, Shiro
Takagi, Satoshi
De Francesco, Francesco
Miyazaki, Motoyasu
Saparov, Arman
Journal Title
Journal ISSN
Volume Title
Publisher
MDPI
Abstract
Background and objectives: Skin grafting is a method usually used in reconstructive surgery
to accelerate skin regeneration. This method results frequently in unexpected scar formations.
We previously showed that cutaneous wound-healing in normal mice is accelerated by a micrograft
(MG) technique. Presently, clinical trials have been performed utilizing this technology; however,
the driving mechanisms behind the beneficial effects of this approach remain unclear. In the
present study, we focused on five major tissue reactions in wound-healing, namely, regeneration,
migration, granulation, neovascularization and contraction. Methods: Morphometrical analysis was
performed using tissue samples from the dorsal wounds of mice. Granulation tissue formation,
neovascularization and epithelial healing were examined. Results: The wound area correlated
well with granulation sizes and neovascularization densities in the granulation tissue. Vascular
distribution analysis in the granulation tissue indicated that neovessels extended and reached the
subepidermal area in the MG group but was only halfway developed in the control group. Moreover,
epithelialization with regeneration and migration was augmented by MG. Myofibroblast is a known
machinery for wound contraction that uses α-smooth muscle actin filaments. Their distribution in
the granulation tissue was primarily found beneath the regenerated epithelium and was significantly
progressed in the MG group. Conclusions: These findings indicated that MG accelerated a series of
wound-healing reactions and could be useful for treating intractable wounds in clinical situations.
Description
Keywords
micrograft, wound-healing, epidermis, granulation, neovascularization, histopathology
Citation
Jimi, S., Takagi, S., De Francesco, F., Miyazaki, M., & Saparov, A. (2020). Acceleration of Skin Wound-Healing Reactions by Autologous Micrograft Tissue Suspension. Medicina, 56(7), 321. https://doi.org/10.3390/medicina56070321