Effect of Roux-en-Y gastric bypass-induced weight loss on the transcriptomic profiling of subcutaneous adipose tissue

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dc.contributor.authorGonzález-Plaza, Juan José
dc.contributor.authorGutiérrez-Repiso, Carolina
dc.contributor.authorGarcía-Serrano, Sara
dc.contributor.authorRodriguez-Pacheco, Francisca
dc.contributor.authorGarrido-Sánchez, Lourdes
dc.contributor.authorSantiago-Fernández, Concepción
dc.contributor.authorGarcía-Arnés, Juan
dc.contributor.authorMoreno-Ruiz, Francisco J.
dc.contributor.authorRodríguez-Cañete, Alberto
dc.contributor.authorGarcía-Fuentes, Eduardo
dc.creatorJuan José, González-Plaza
dc.date.accessioned2018-01-04T11:00:51Z
dc.date.available2018-01-04T11:00:51Z
dc.date.issued2016-02-01
dc.description.abstractAbstract BackgroundThe changes in the transcriptomic profiling of subcutaneous adipose tissue (SAT) when weight loss stabilizes after a Roux-en-Y gastric bypass (RYGB) are still largely unknown. ObjectivesTo investigate the changes produced in SAT gene expression of morbidly obese women when their weight loss stabilizes 2 years after RYGB. SettingUniversity hospital. MethodsSAT biopsies of the periumbilical area were taken before and 2 years after RYGB. Gene expression levels were assessed by microarray analysis and significant differences in gene expression were validated by real-time quantitative polymerase chain reaction. The findings were also confirmed in an independent population of morbidly obese women. ResultsMicroarray analysis revealed that the overexpressed differentially expressed genes have a prominent role in the pathways involved in biosynthetic processes, especially lipid or carboxylic ones (stearoyl-Coenzyme A desaturase-1, fatty acid desaturase-1, fatty acid elongase-6, ATP citrate lyase, fatty acid synthase, lipin-1, monoacylglycerol O-acyltransferase, patatin-like phospholipase domain containing-3, phosphate cytidylyltransferase-2, cholesteryl ester transfer protein, transmembrane 7 superfamily member 2, pyruvate carboxylase, and glycogen synthase 2). Most of the underexpressed differentially expressed genes are related with immune system and inflammation processes (immune responses, response to stress, cell death, regulation of biological quality, immune effector process, the response to endogenous stimulus, and the response to other types of stimulus). ConclusionAn improvement of the SAT inflammatory and immune profile and an induction of genes involved in the regulation of lipid metabolism are shown when weight loss stabilizes 2 years after RYGB. Most of the genes shown are clearly linked to obesity and other metabolic disorders.
dc.identifierDOI:10.1016/j.soard.2015.07.007
dc.identifier.citationJuan José González-Plaza, Carolina Gutiérrez-Repiso, Sara García-Serrano, Francisca Rodriguez-Pacheco, Lourdes Garrido-Sánchez, Concepción Santiago-Fernández, Juan García-Arnés, Francisco J. Moreno-Ruiz, Alberto Rodríguez-Cañete, Eduardo García-Fuentes, Effect of Roux-en-Y gastric bypass-induced weight loss on the transcriptomic profiling of subcutaneous adipose tissue, In Surgery for Obesity and Related Diseases, Volume 12, Issue 2, 2016, Pages 257-263
dc.identifier.issn15507289
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S1550728915002920
dc.identifier.urihttp://nur.nu.edu.kz/handle/123456789/3108
dc.relation.ispartofSurgery for Obesity and Related Diseases
dc.rights.licenseCopyright © 2016 American Society for Bariatric Surgery. Published by Elsevier Inc. All rights reserved.
dc.subjectTranscriptomics
dc.subjectMicroarray
dc.subjectBariatric surgery
dc.subjectGastric bypass
dc.subjectSubcutaneous adipose tissue
dc.subjectMorbid obesity
dc.subjectFunctional annotation
dc.subjectExpression analysis
dc.titleEffect of Roux-en-Y gastric bypass-induced weight loss on the transcriptomic profiling of subcutaneous adipose tissue
dc.typeArticle
dcterms.publisherSurgery for Obesity and Related Diseases
elsevier.aggregationtypeJournal
elsevier.coverdate2016-02-01
elsevier.coverdisplaydateFebruary 2016
elsevier.endingpage263
elsevier.identifier.doi10.1016/j.soard.2015.07.007
elsevier.identifier.eid1-s2.0-S1550728915002920
elsevier.identifier.piiS1550-7289(15)00292-0
elsevier.identifier.pubmedid26615868
elsevier.identifier.scopusid84947968311
elsevier.issue.identifier2
elsevier.openaccess0
elsevier.openaccessarticlefalse
elsevier.openarchivearticlefalse
elsevier.startingpage257
elsevier.teaserThe changes in the transcriptomic profiling of subcutaneous adipose tissue (SAT) when weight loss stabilizes after a Roux-en-Y gastric bypass (RYGB) are still largely unknown....
elsevier.volume12

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