(Journal of Acupuncture and Meridian Studies, 2009-04-01) Yoo, Jung Sun; Kim, Hong Bae; Ogay, Vyacheslav; Lee, Byung-Cheon; Ahn, Saeyoung; Soh, Kwang-sup
Objective: The present study has been designed to find a possible new route for the
metastasis of cancer cells on the fascia surrounding tumor tissue using a novel technique
of trypan blue staining.
Materials and Methods: Tumor tissues were grown in the skin of nude mice after subcutaneous
inoculation with human lung cancer cells. Trypan blue was recently identified
as a dye with specificity for Bonghan ducts (BHDs) and not other tissues, such as
blood or lymph vessels or nerves.
Results: We demonstrate that the trypan blue staining technique allows the first
visualization of BHDs which are connected to tumor tissues.