Biology

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Browsing Biology by Author "Assadian, Ojan"

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    Acceptability and tolerability of liquid versus gel and standard versus virucidal alcohol-based hand rub formulations among dental students
    (American Journal of Infection Control, 2013-11-01) Stauffer, Fritz; Griess, Marion; Pleininger, Gabriele; Zhumadilova, Anara; Assadian, Ojan; Fritz, Stauffer
    BackgroundHand hygiene is effective to prevent the transmission of microorganisms in health care settings, but compliance remains low, even when easy access to hand cleaning agents is provided. ObjectiveFormulation of alcohol-based hand rub (ABHRs) may influence staff compliance to hand hygiene. The aim of this prospective longitudinal study (1 week) was to investigate possible differences of 4 different gel or liquid ABHR formulations, with or without virucidal claim among dental students. MethodsParticipants were randomly assigned to dental treatment cubicles, equipped with either a gel or a liquid based ABHRs, with our without a virucidal claim. Participants assessed the subjective acceptability and the tolerability of test formulations on their hands over a period of 1 week using the 14 item, 7-point Lickert scale World Health Organization questionnaire. ResultsAll tested ABHRs passed the subjective acceptability criteria of ≥50% above 4 for the items “color and fragrance” and for all other items of >75% above 4 and may be regarded as “good.” Significant differences were observed between the 2 gels but not between the 2 liquid ABHRs. For subjective skin tolerability, no significant difference was observed between the liquid formulations after 1 consecutive week of application. However, the difference between the 2 gels was highly significant. ConclusionVirucidal ABHR formulations may be better accepted and tolerated over prolonged periods by dental students than anticipated. The user acceptability of ABHRs depend more on the specific product's formula than its general category.
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    Proposal for assessment of the antimicrobial efficacy of undiluted medical honey: Using a standardized phase 2/step 2 in vitro stainless steel disc carrier test model
    (Wound Medicine, 2013-07-01) Goerdt, Anna-Maria; Assadian, Ojan; Razavi, Behzad; Igelbrink-Holter, Dorothee; Simon, Arne; Hübner, Nils-Olaf; Partecke, Lars Ivo; Zhumadilova, Anara; Heidecke, Claus-Dieter; Kramer, Axel; Anna-Maria, Goerdt
    Abstract PurposeThe antimicrobial efficacy of medical honeys used for professional wound care was published previously. So far, all in vitro antimicrobial tests performed have been conducted using non-standardized methods and used diluted medical honey. A standardized, reproducible phase 2/step 2 in vitro disc carrier model is proposed allowing testing the antimicrobial efficacy of undiluted medical honey. MethodsUsing a standardized disc carrier model, the log10 Reduction Factor (RF) of undiluted medical honey was determined for MSSA, MRSA, VRE, Pseudomonas aeruginosa, and Candida albicans, with and without protein challenge. ResultsAn antimicrobial efficacy was observed after 1h. Without organic challenge, log10RF >5 within 24h was achieved for all tested microorganisms, except for Staphylococcus aureus (log10RF: 4.8/24h). Challenged with 10% bovine serum albumin, a similar pattern was observed at 24h. The most susceptible organism was C. albicans (log10RF of >3/5min). Over an exposure time of 48h, the tested medical honey's antimicrobial activity under protein challenge achieved log10RFs >6 for C. albicans and >7log10 for all tested bacteria. ConclusionsIt was demonstrated that the proposed standardized phase 2/step 2 in vitro stainless steel disc carrier test model is applicable for testing the antimicrobial activity of highly viscous and undiluted compounds such as medical honey. Undiluted medical honey exhibits a strong antimicrobial efficacy even under protein challenge after 1h, which increases to >6log10 for C. albicans, and >7log10 for Gr+ and Gr− bacteria.